Guppy_basecaller -i fast5s/ -r -s fast5s_guppy \ # Download and unzip the example data and pre-trained models. Install deepsignal-plant, its dependencies, and other required packages in one step using conda and environment.yml: Guppy and tombo are required to basecall and re-squiggle the raw signals from nanopore reads before running deepsignal-plant. ContentsÄeepsignal-plant is built on Python3 and PyTorch. References: issue #8, tombo issue #254, and vbz_compression issue #5. Tar zxvf ont-vbz-hdf-plugin-1.0.1-Linux-x86_64.tar.gzÄ®xport HDF5_PLUGIN_PATH=/abslolute/path/to/ont-vbz-hdf-plugin-1.0.1-Linux/usr/local/hdf5/lib/plugin download ont-vbz-hdf-plugin-1.0.1-Linux-x86_64.tar.gz (or newer version) and set HDF5_PLUGIN_PATH # Sudo apt-get install libhdf5-serial-dev hdf5-tools ![]() ![]() install hdf5/hdf5-tools (maybe not necessary) # ubuntu
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